Extended Data Fig. 1: Comparison of gut microbiota based on subjects-related variates.
From: Chronic liver disease enables gut Enterococcus faecalis colonization to promote liver carcinogenesis
(a) Chao1 was calculated from metagenomic taxonomy data. Boxes represent 25th-75th percentile of the distribution. The median is shown in the middle of the boxes. Whiskers represent min to max. The number of samples were 21–24 and 10 iterations were performed in the analysis. (b–h) Unweighted UniFrac distances were calculated with taxonomic data for fecal samples based on subjects-related variates including age (b), use of PPI/H2RA (c), BCAA (d), obesity (e), daily alcohol consumption (f), smoking (g) and cirrhosis (h). Distances were visualized by principal coordinates analysis. Significant differences between groups were determined by PERMANOVA, and P values and q value are shown at the bottom of the plots. (a-b) LDA was performed with relative abundance of taxa in the feces. (i) HD subjects (n=24) and CLD/HCC subjects (n=23) without treatment of PPI, H2RA or BCAA were compared by LDA. (j) Ages were matched between HD subjects (n=13) and CLD/HCC subjects (n=36) at over 55 years old and compared by LDA. LDA scores with P value < 0.05 are shown in the graphs. q, adjusted P value by false discovery rate method; PPI, proton pump inhibitors; H2RA, H2 receptor antagonists; BCAA, branched-chain amino acids. (a), Kruskal Wallis test;. *, P < 0.05; **, P < 0.01. Exact P-values were as follows: (a) HD vs. CLD, P>0.999; HD vs. HCC, P=0.0045; CLD vs. HCC, P=0.0143.
