Extended Data Fig. 10: Gating Strategy used for flow cytometry. | Nature Cancer

Extended Data Fig. 10: Gating Strategy used for flow cytometry.

From: RNF2 ablation reprograms the tumor-immune microenvironment and stimulates durable NK and CD4+ T-cell-dependent antitumor immunity

Extended Data Fig. 10: Gating Strategy used for flow cytometry.The alt text for this image may have been generated using AI.

a, Gating Strategy used for analysis of immune cells from tumors isolated from tumor-bearing mice presented on Fig. 2a–e, Fig. 3a, b, Fig. 4c, Fig. 7e, Fig. 8e, Extended Data Fig. 3v, 5a, c, g, h, Extended Data Fig. 9c. b, Gating Strategy used for analysis of each subset of CD4+ T-cells in a (5-2). c, Gating Strategy used for analysis of each subset or marker of NK cells in a (5-3). d, Gating Strategy used for analysis of each subset or marker of CD45− tumor cells in a. e, Gating Strategy used for analysis of CD4+ T-cells or NK cells from the in vitro co-culture assays presented on Fig. 3c, d, f, g, Fig. 4j, k, Extended Data Fig. 5b, d, e, Extended Data Fig. 5i–j, p, s. f, Gating Strategy used for analysis of dead tumors in the in vitro killing assays presented on Fig. 3e, Fig. 4l, Extended Data Fig. 5f. g, Gating Strategy used for sorting of tumor cells (CD45−) and NK cells (CD45+NKp46+CD3−) for RNA-Seq and ATAC-Seq presented on Fig. 4a, b, Fig. 5a–f, Fig. 6, Fig. 7f-j, Extended Data Figs.4c,d, 6, 7a, c–f, 8a–c.

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