Extended Data Fig. 7: NR2F2 loss of function characterization in skin human SCC cell lines.
a: WB for NR2F2 and β-actin in A431 control and NR2F2 shRNA KD #1 and #2 human cell lines. b: NR2F2 knockdown alters the growth of spheroids of A431 skin SCC cell lines. Pictures were taken 4 and 11 days after seeding. Representative images of 3 independent experiments. c, d: Wound-healing assay of NR2F2 shRNA KD #1 (c) and #2 (d) following doxycycline induction. Scale bar=500 µm. Representative images of 3 independent experiments. e: qRT-PCR of upregulated and downregulated genes following NR2F2 KD and CIA1 exposure in A431 cell line (n=3 technical replicates). Data are represented as mean ± s.e.m. f: WB for NR2F2, HA and β-actin in A431 NR2F2 GOF human cell line (A431 WT and 3HA-NR2F2 GOF). g: Overlap of the A431 NR2F2 ChIP-seq peaks with the downregulated and upregulated genes in the NR2F2 KD#2 cell line (p-values are calculated using a Two-sided hypergeometric test). h: Overlap of the A431 NR2F2 ChIP-seq peaks with the downregulated and upregulated genes in the NR2F2 KO mouse SCC. p-values are calculated using a Two-sided hypergeometric test. WBs are representative of two independent experiments with similar results in f, and three independent experiments with similar results in a.
