Extended Data Fig. 2: GFAP staining confirms that astrocytes are stripped from vessels upon activation of micrometastases.
From: Astrocytic laminin-211 drives disseminated breast tumor cell dormancy in brain
a) Representative Z-projections of disseminated eGFP-T4-2 cells and astrocyte (GFAP+, cyan) coverage of DTC-associated vessels in the brains of NOD SCID mice across four stages of brain metastatic progression, 8 weeks following intracardiac inoculation. Yellow arrows indicate Ki67+ tumour cells (see inset for CD31 and GFAP channels only). Scale bar = 20 µm. From left to right, images are representative of n = 12, 5, 6 and 12 independent lesions imaged across n = 3 mice. b) Quantification of astrocyte vessel coverage across metastatic progression. P-values calculated for n = 35 lesions across 3 mice by one-way ANOVA and Turkey’s post hoc test. Centre line represents the mean, and error bars the s.e.m.
