Extended Data Fig. 6: MTHFD2i induce differentiation of AML blasts.
(a) Flow cytometry analysis of CD11b expression in HL-60 cells after 96 h incubation with TH7299 3 μM, TH9028 50 nM, ATRA 1 μM, or ATRi VE-821 1 μM, in presence or absence of thymidine 5 μM supplementation. To the left, representative histograms, and to the right, bar graphs showing the percentage of CD11b positive cells, displayed as means, n = 2. *** P < 0.001; one-way ANOVA (F = 1983, DF = 9). Shown is a representative out of two independent experiments. (b) CD11b/PI live staining of HL-60 cells upon incubation with ATRA 1 μM, TH7299 5 μM or TH9619 50 nM, assessed at each indicated time point. Approximately 20,000 events were gated per condition. Bar graphs represent the percentage of double-negative, single positive and double-positive populations as means ± SD. Shown is a representative experiment out of two independent experiments conducted in triplicate.
