Extended Data Fig. 4: Patient-derived model growth rate and proliferation comparison.
PDxO xenograft (PDxoX) tumor volumes (a) and tumor growth rates (b) compared to parental PDX lines for HCI-001, HCI-002, HCI-010, HCI-025, and HCI-027. PDxOs were in culture for 51-64 days (PDxoX early) or 113-123 days (PDxoX late) prior to xenografting. Tumor volume shown as mean ± s.e.m. Tumor growth rates shown as mean ± s.e.m. HCI-001 parental n=5 mice, early n=5 mice, late n=4 mice; HCI-002 parental n=5 mice, early n=5 mice, late n=4 mice; HCI-010 parental n=1 mouse, early n=6 mice; HCI-025 parental n=5 mice, early n=5 mice, late n=4 mice; HCI-027 parental n=7 mice, early n=5 mice, late n=4 mice, late V2 n=4 mice, PDX parental P1 n=5 mice. Statistical comparisons to control (PDX parental) for growth rates by ordinary one-way ANOVA, Tukey’s multiple comparisons test with a single pooled variance. HCI-010 PDX only generated n=1 mouse, requiring use of two-tailed unpaired t test. (c) Quantification of Ki67+ nuclei of parental PDX, early (51-68 days) and late (113-127 days) PDxO cultures and early and late PDxoX. Ki67 staining was performed on one PDX tumor per HCI line, and n=3 individual sets of IHC stains were performed. Data are shown as mean ± s.e.m. and normalized to total hematoxylin+ nuclei count for each image. Statistical comparisons to control (PDX) for Ki67 quantification by ordinary one-way ANOVA, Tukey’s multiple comparisons test with a single pooled variance.
