Extended Data Fig. 8: Open-reading frames of XPR1 resistant to CRISPR/Cas9-mediated gene editing. | Nature Cancer

Extended Data Fig. 8: Open-reading frames of XPR1 resistant to CRISPR/Cas9-mediated gene editing.

From: Phosphate dysregulation via the XPR1–KIDINS220 protein complex is a therapeutic vulnerability in ovarian cancer

Extended Data Fig. 8

a) Immunofluorescent localization of XPR1 mutants using the V5 epitope tag. Left, WT XPR1 localizes to the secretory pathways as well as puncta within the cytoplasm. Middle, XPR1 (short) staining appears more diffuse. Note similar localization patterns between L218S and wildtype XPR1 alleles. Scale bar = 200 µm. N = 1 experiment representative of N = 2 independent transductions. b) Western blot validation of guide-resistant ORF. OVISE.Cas9 cells (parental, left, or overexpressing the WT XPR1 ORF, right, used in Fig. 3e) were infected with the indicated sgRNA and harvested 5 days after infection. The XPR1 ORF includes a mutation to block binding of sgXPR1_2 but not sgXPR1_1. Note the inactivation of both endogenous and overexpression ORF with sgXPR1_1 and only endogenous XPR1 with sgXPR1_2. N = 1 experiment representative of N = 2 independent transductions.

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