Extended Data Fig. 2: Activation of AKT signaling is a specific consequence of XPO1 inhibition.

a) Immunoblot depicting protein levels of phosphorylated PRAS40, FOXO3a, and TSC2 following 24-hour treatment of OCI-AML2 cells with selinexor. b) Immunoblot depicting protein levels of phosphorylated AKT at T308 and S473 following 24-hour treatment of OCI-AML2 and MOLM-13 cells with eltanexor. c) Immunoblot depicting protein levels of phosphorylated AKT at T308 and S473 in OCI-AML2 cells treated with a panel of standard-of-care therapies for 24 hours. Representative immunoblots of n = 2-3 independent experiments yielding similar results. B-actin included as loading control.