Fig. 3: A correlated program of CRS-related cytokines is frequently upregulated after COVID-19 vaccination under immune-checkpoint therapy.
a, Heat map indicating Pearson correlation indices of log10(fold change) cytokine concentrations after COVID-19 vaccination from n = 35 patients. Colored bars on the sides indicate clusters obtained from hierarchical clustering. b, Heat map indicating probability of each pair of cytokines clustering together as calculated by bootstrapping (n = 10,000 samplings) from n = 35 patients. Colored bars on the sides indicate clusters obtained from hierarchical clustering. c, Heat maps indicating log10(fold change) of cytokine concentrations after vaccination. Bar graph on the side indicates average log10(fold change) of cytokines in each row with concentrations after the first or second vaccination dose labeled according to the color code on the right from n = 35 patients. P values (two-sided) were calculated using a Wilcoxon signed-rank test (test statistics from left to right are 231, 425, 301 and 270; Cohen’s D calculated as mean fold change/s.d. fold change from left to right is 0.93, 3.22, 1.32 and 1.21). P values significant (α = 0.05) after correcting for multiple comparisons with the Benjamini–Hochberg method are indicated with an asterisk. d, Line-plots indicating cytokine concentrations of cytokine program 1 cytokines after vaccination from n = 35 patients and vaccine type indicated in the color code at the bottom right.
