Extended Data Fig. 7: Analysis of transcriptional cell states during chemotherapy and after recovery in AKP MTO cultures.
From: Mex3a marks drug-tolerant persister colorectal cancer cells that mediate relapse after chemotherapy
a, Experimental design b-e Violin plots for key marker genes of b, Lgr5 ISCs; c, Fetal progenitors/revival stem cells; d, YAP target genes; and e, proliferative cells. Expression in both tdTomato+ and tdTomato- cells after FOLFIRI treatment and recovery are shown. f-g, UMAP representation of untreated MTOs (NT) and sorted tdTomato+ and tdTomato- cells 4d after FOLFIRI treatment and upon recovery (30d). Note the overlapping distribution of tdTomato+ and tdTomato- cells. h-m, Mex3a levels or average expression of the indicated gene signatures in individual tdTomato+ cells represented in UMAPs (left panels). Signatures are detailed in Supplementary Table 6. Violin plots (right panels) compare expression levels in tdTomato+ and tdTomato- cells after FOLFIRI treatment and upon recovery.
