Extended Data Fig. 4: BMP4 prolongs the survival of DIPG xenograft model mice.
(a) Schematic for the construction of a xenograft mouse model upon orthotopic injection of 1 ×105 BMP4-pretreated (50 ng/mL) 24 hr or untreated DIPG cells. Created with BioRender.com. (b) The normalized bioluminescence activity in TT150630 mouse model was plotted and the statistical difference between BMP4 pre-treated and vehicle treated groups was significant (n = 6 mice in each group). (c) The normalized bioluminescence activity in SU-DIPG17 mouse model was plotted and the statistical difference between BMP4 pre-treated and vehicle treated groups was significant (n = 6 mice in each group).(d) Kaplan–Meier analysis from animals implanted with TT150630 cells with indicated treatment (n = 6 mice in Ctr group vs n = 5 mice in BMP4 pretreatment group) in the pons. Log-rank test was performed. (e) Representative images of pons from animals implanted with SU-DIPG17 cells with or without BMP4 treatment analyzed by H&E staining. Regions marked by the box are magnified below. Scale bars, 1,000 µm (top), 50 µm (middle) and 20 µm (bottom). (f) Immunofluorescence of pons section from animals implanted with SU-DIPG17 cells with or without BMP4 treatment for anti-human nuclear antigen (HNA). Scale bars, 1,000 µm (top), 50 µm (middle) and 20 µm (bottom). For b and c, boxplots define the interquartile range (IQR) split by the median, with whiskers extending to the most extreme values within 1.5 × IQR beyond the box, statistical significance was calculated by two-tailed unpaired Student’s t-test. The experiments in e and f have been repeated three times with similar results.
