Extended Data Fig. 6: Glycolysis and the TCA cycle were slightly affected by nucleosides under low glucose conditions.

(a, b) 14837 T cells were cultured in complete media (25 mM glucose), low glucose media (1 mM glucose) with or without uridine (U, 0.5 mM), inosine (I, 0.5 mM), adenosine (A, 0.5 mM), guanosine (G, 0.5 mM), cytidine (C, 0.5 mM) for 48 h. The cells were counted to calculate the cell proliferation (a). Metabolic analysis of cells was performed by mass spectrometry (b). Data are shown as mean ± SD. (n = 3 biologically independent samples). Statistical analysis was performed using ordinary one-way ANOVA with Tukey’s multiple comparisons test.