Extended Data Fig. 2: LNCaP/AR-sgTP53/RB1 is a highly resistant and lineage plastic cell line model.
a, Western Blot represents the level of RB1, TP53, JAK1 and ACTIN proteins in a series of human PCa cell line models; representative pictures of 2 repeats with similar results were shown. b, Fluorescence microscope imaging shows the cell mixtures of sgTP53/RB1-RFP cells (red) and sgNT-GFP cells (green) on Day 0 and Day 8 of the competition assay cultured in CSS medium and 10 µM Enz; representative pictures of n = 4 independently treated cell cultures were shown. c, Relative cell number fold change of LNCaP/AR cells transduced with Cas9 and annotated guide RNAs measured in the competition assay. The cell mixtures of sgNT-GFP and sgTP53/RB1-RFP were treated in CSS medium with 10 µM Enz for 8 days and the number of GFP/RFP positive cells were measured by FACS on Day 0, 4 and 8. n = 4 independently treated cell cultures. d, Relative expression of canonical lineage marker genes in LNCaP/AR cells transduced with Cas9 and annotated guide RNAs. For panel (c-d), p-values were calculated using two-tailed multiple t-test with Welch’s correction and annotated in figure. e, Western Blot represents the level of RB1, TP53, JAK1, p-STAT1 and ACTIN proteins in LNCaP/AR cells transduced with Cas9 and annotated guide RNAs; representative of 2 repeats with similar results were shown. f, Relative expression of canonical JAK–STAT signaling and lineage marker genes in LNCaP/AR mCRPC cells transduced with Cas9 and annotated guide RNAs. p-values were calculated using two-way ANOVA with Bonferroni multiple-comparison test and annotated in figure. For all panels unless otherwise noted, n = 3 independently treated cell cultures and mean ± s.e.m. is represented.
