Extended Data Fig. 8: Production of mCCL5 and oHSV in the GBM TME.
(a) Cmab-mCCL5 production in the brains collected from GBM mice treated with the indicated different doses of OV-Cmab-mCCL5. Mice were sacrificed on day 3 post treatment. Error bars indicate s.d., and statistical analyses were performed by one-way ANOVA with P values corrected for multiple comparisons by Bonferroni method (n = 5 mice in the saline and 2 × 105 PFU groups and n = 4 mice in the 1 × 105 PFU and 4 × 105 PFU groups). (b, c) Immunohistochemical (IHC) analysis of mCCL5, oHSV, and H&E staining of the brains collected from mice treated with saline, OV-Q1, or OV-Cmab-mCCL5. Slides with brain tissue isolated from the experimental mice were subjected to H&E and IHC staining, and anti-HSV antibody and anti-mCCL5 antibody are used for detecting mCLL5 production and oHSV (OV-Q or OV-Cmab-mCCL5) existence, respectively. Images with high and low magnification are shown in (b, scale bars, 100 μm) and (c, scale bar, 2 mm), respectively. The boxed images in (c) are shown at higher power in (b).
