Fig. 1: CD47 blockade enhances local tumor inhibition following irradiation of SCLC tumors.
From: Radiotherapy in combination with CD47 blockade elicits a macrophage-mediated abscopal effect
a, Growth curves of KP1 SCLC allografts in immunodeficient NSG mice with the indicated treatments. n = 4 (RT + anti-CD47) or n = 5 (control, anti-CD47 and RT) mice. ****P < 0.0001, *P = 0.0159. b, Tumor-infiltrating macrophages (CD11b+F4/80+) identified by flow cytometry from tumors collected in a. c, Quantification of tumor-infiltrating macrophages from b. Data are representative of n = 2 independent experiments. n = 3 (RT + anti-CD47) or n = 5 (control, anti-CD47 and RT) tumors. **P = 0.0079, *P = 0.0320, ****P = 0.0002. d, CD47 expression for KP1 or KP1 Cd47 knockout cells by flow cytometry. e, Growth curves of KP1 control and Cd47 knockout allografts in NSG mice. f, Quantification of tumor volume 4 d after radiation. n = 1 experiment with n = 3 tumors per condition. *P = 0.0170, *P = 0.0279. g, Growth curves of KP1 SCLC allografts in B6129SF1 immunocompetent recipient mice with the indicated treatments. n = 1 experiment with n = 3 (RT + anti-CD47) or n = 4 (control, anti-CD47 and RT) tumors. h, Growth curves of KP1 control and Cd47 knockout SCLC allografts in immunocompetent syngeneic mice. Tumors were irradiated at two different time points to account for the slower growth of Cd47 knockout cells. i. Quantification of tumor volume 8 d after radiation. n = 1 experiment with n = 4 tumors. *P = 0.0286, *P = 0.0390. Two-tailed Student’s t-tests following two-way analysis of variance (ANOVA) were performed in a (P < 0.0001) and g (P < 0.0001). Two-tailed Student’s t-tests following one-way ANOVA were performed in c (P = 0.001), f (P = 0.0006) and i (P < 0.0001). Error bars represent s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
