Extended Data Fig. 3: Enrichment of NF-kB signatures, death receptor related gene expression upon senescence induction and extrinsic apoptosis priming in senescent cells.
From: cFLIP suppression and DR5 activation sensitize senescent cancer cells to senolysis
a-f, Transcriptomic analysis of death receptor genes TNFSF10 (TRAIL), TNFRSF10C (DCR1), TNFRSF10D (DCR2), TNFRSF10A (DR4), TNF and TNFRSF1A (TNFR1) from 13 cell lines made senescent by etoposide and alisertib (ArrayExpress E-MTAB-9970). g, Real-time PCR analysis of DR4, DR5, cFLIP, TRAIL in SK-Hep1 cells treated with 0.5 µM alisertib. h, Real-time PCR analysis of TRAIL and DR5 in cFLIP knockout SK-Hep1 cells with shRNAs against these genes. i, Cell viability assessed by colony formation assay in the cFLIPKO cells with shRNAs against TRAIL or DR5 and treated with 0.5 µM alisertib for 7 days. Error bars in this figure panel g-i represent as mean ± standard deviations, N = 3 independent experiments, statistical significance was calculated by two-tailed t-test.
