Extended Data Fig. 5: Death receptor agonist-mediated senolysis in additional models induced by different senescence inducers.
From: cFLIP suppression and DR5 activation sensitize senescent cancer cells to senolysis
a, Caspase-3/7 green apoptosis assay on alisertib-induced senescent SK-Hep1 cells treated with 100 ng/ml Lz-TRAIL. b, Cell viability accessed by Colony formation in the alisertib-induced senescent and proliferating SK-Hep1 cells treated with 100 ng/ml Lz-TRAIL, 10 µM Z-VAD-FMK. c, Dose-response curve of 0.5 µM alisertib-induced senescent and proliferating SK-Hep1 cells treated with conatumumab (The result represents at least 3 repeats with similar results). d, Colony formation assay in drug-induced (one week of 1 µM Barasertib, 100 nM CFI-400945, 2 µM Etoposide treatment) senescent SK-Hep1 cells treated with conatumumab. e, Senescence-associated with beta-galactosidase staining in 7 days post-IR 10 Gy on SK-Hep1 cells. The black scale bars present 100 µm. f-g, Colony formation (f) and Western blot of cl-PARP (g) in the IR-induced senescent and proliferating SK-Hep1 cells treated with 1 µg/ml conatumumab and 10 µM Z-VAD-FMK. h, Cell viability accessed by colony formation in the IR-induced senescent and proliferating SK-Hep1 cells treated 100 ng/ml Lz-TRAIL. i, Senescence associated with beta-galactosidase staining in 7 days 10 Gy post-ionizing irradiated (IR) A549 cells. The black scale bars present 100 µm. j-k, Colony formation assay (j) and western blot of cl-PARP (k) in the 10 Gy IR-induced senescent and proliferating A549 cells treated with 100 ng/ml Lz-TRAIL and 10 µM Z-VAD-FMK. l, Cell viability accessed by colony formation of 0.5 µM alisertib-induced senescent and 10 µg/ml LPS cultured cells treated with the 4 µg/ml conatumumab or 200 ng/ml Lz-TRAIL. m, Real-time PCR of DR5, cFLIP, TRAIL in in LPS treated and alisertib-induced senescent A549 cells. The results in panel g, k are representative of at least 3 repeats with similar results. Error bars in this figure panel a, b, d-f, h-j, l, m represent as mean ± standard deviations, N = 3 independent experiments, statistical significance was calculated by two-tailed t-test.
