Extended Data Fig. 3: Co-Culture Metabolic Assays. | Nature Cancer

Extended Data Fig. 3: Co-Culture Metabolic Assays.

From: Differential integrated stress response and asparagine production drive symbiosis and therapy resistance of pancreatic adenocarcinoma cells

Extended Data Fig. 3

A. Fluorescently labeled clone K co-cultures were treated with oligomycin or vehicle. B. Representative images from Fig. 2D of labeled K co-cultured with unlabeled resistant clones (H,V) or with unlabeled sensitive clones (K,M). C. Fluorescently labeled clone N co-cultures were treated with oligomycin, phenformin or vehicle. D,E. Representative images of 1 nM oligomycin or vehicle treated co-cultures with unlabeled resistant V (D), or unlabeled sensitive clones N (E) to generate data presented in Fig. 2E. F. Fluorescently labeled clone V co-cultures were treated with 1.5 nm oligomycin or vehicle. G. Representative images from Fig. 2F of labeled V co-cultured with unlabeled resistant clones (V,H) or with unlabeled sensitive clones (N,M). Error bars are mean ± SD, Scale Bars = 400 µm, * P ≤ 0.05 by one-way Anova with Tukey post hoc.

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