Extended Data Fig. 7: Interrogation of Human PDA Clonality.

A. Immunostaining for ASNS in human PDA patient tissues, dashed boxes magnified in insets. Scale bars = 100 µm. B. Uniform Manifold Approximation and Projection (UMAP) representation of 3 epithelial cell clusters identified from single cell RNA analysis of a tumor biopsy of PDA Patients 1238 and 1314, with non-hierarchical clustering analysis of epithelial clusters across a set of integrated stress response (ISR) genes. C. Representative images from Fig. 5H. Clonal line PATC53-23 was encoded with a fluorescent label, plated in direct co-culture with unlabeled clones PATC53-23 or PATC53-34, then treated with 0.5 nM oligomycin or 125 µM phenformin relative to vehicle control. Scale bar = 1 mm.