Extended Data Fig. 8: Impacts of Asparagine Rescue on Metabolism.
A. Immunoblot analysis of Vinculin and Cleaved Caspase 3 across 1 nM treated oligomycin group 1 clones (E,V,H) vs. group 2 (K,M,N,T). Staurosporine treated clonal line N is included as a positive control. B. Non-hierarchical clustering heatmap of metabolites that differed between experimental conditions (p = 0.1) from clone N cells treated with asparagine, oligomycin, asparagine + oligomycin, or vehicle 4 hours post treatment (n = 3). C-H. Isotopic labeling patterns in clones N and V using media supplemented with universally-labeled 13C-asparagine (Asn), 13C-glucose, or 13C-glutamine, relative to unlabeled controls, for asparagine (C, n = 3), aspartate (D, n = 3), citrate (E, n = 3), or uridinylates (F-H, n = 3). I. Isotopologue abundance of aspartate from clone N pre-labeled with universally labeled 13C-glutamine, then switched to media containing unlabeled-glutamine and treated with asparagine, oligomycin, asparagine + oligomycin, or vehicle for 4 hours (n = 3). Error bars are mean ± SD.
