Extended Data Fig. 1: Enriched Metabolic Pathways Present in Clonal Populations. | Nature Cancer

Extended Data Fig. 1: Enriched Metabolic Pathways Present in Clonal Populations.

From: Differential integrated stress response and asparagine production drive symbiosis and therapy resistance of pancreatic adenocarcinoma cells

Extended Data Fig. 1

A. Agarose gel visualization of PCR products for recombination of KrasG12D allele in clonal lines vs. KrasLSL-G12D/+ mouse tail control DNA. B. Top 50 pathways identified by MetaboAnalyst among metabolites differentially represented between group 1 and group 2 clones. C. Relative abundance of metabolites from glycolysis between clonal populations (n = 3 replicates per cell line). F-1,6-BP = fructose 1,6-bisphsophate, GA3P = glyceraldehyde-3-phosphate, DHAP = dihydroxyacetone phosphate. D. Lactate production of clonal cell lines (n = 3 replicates per cell line). E. Relative abundance of glutamine and glutamate between clonal populations (n = 3 replicates per cell line). F. Amniooxyacetic acid (AOA) dose response curves used to generate IC50 values in Fig. 1D. G. Oligomycin dose response curves used to generate IC50 values in Fig. 1E. H. Phenformin dose response curves used to generate IC50 values in Fig. 1F. Each line represents an independent dose response analysis. Error bars are mean ± SD, *** P ≤ 0.001; **** P ≤ 0.0001 by two-tailed Mann–Whitney test.

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