Extended Data Fig. 2: Clonal Metabolic Phenotypes.
A. Citrate Synthase (CS) activity of clonal lines (n = 3 per clone). B. MitoStress profile of the oxygen consumption rate (OCR) over time, treated with (2 μM), FCCP (0.5-2 μM), and rotenone/antimycin A (1 μM) at the indicated time points. Data are average of technical replicates normalized to independent experiments for each clone (n = 4 clone V, n = 4 clone E, n = 3 clone H, n = 5 clone N, n = 4 clone K, n = 2 clone M, n = 4 clone T). C. Metabolic phenotypes of clonal cell lines defined as the ratio of the OCR to extracellular acidification rate (ECAR) (n = 4 clone V, n = 4 clone E, n = 3 clone H, n = 4 clone K, n = 2 clone M, n = 5 clone N, n = 4 clone T). D. Basal ECAR of clonal lines (n = 4 clone V, n = 4 clone E, n = 3 clone H, n = 4 clone K, n = 2 clone M, n = 5 clone N, n = 4 clone T). E. Basal OCR of clonal lines (n = 4 clone V, n = 4 clone E, n = 3 clone H, n = 4 clone K, n = 2 clone M, n = 5 clone N, n = 4 clone T). F. Metabolic fitness as defined by the spare respiratory capacity of clonal cell lines (n = 4 clone V, n = 4 clone E, n = 3 clone H, n = 4 clone K, n = 2 clone M, n = 5 clone N, n = 4 clone T). G. Histogram representation of the MitoTracker Red intensity of group 1 clones (H,V) and group 2 clones (T,N). H. Representative images of MitoTracker Red staining at the mean fluorescence index (MFI) of I. Scale bar = 7 µm. J. Histogram representation of the MitoTracker Green intensity of group 1 clones (H,V) and group 2 clones (T,N). K. Doubling times of the individual clone lines (n = 3 per clone). Error bars are mean ± SD, * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001 by two-tailed Mann–Whitney test.
