Extended Data Fig. 2: Serial patient CD34 + scRNA sequencing shows elevated DUSP6 along MPN to sAML progression.
From: DUSP6 mediates resistance to JAK2 inhibition and drives leukemic progression
a) Gene set enrichment analysis of top altered Hallmark pathways in serial CD34 + samples at the sAML stage compared to MPN stage of three patients. b) Violin plots showing relative expression of top shared differentiate expressed genes in sAML vs MPN disease states from three patients, and healthy donors (N34, N39). c) Venn diagram showing shared candidates identified in the top 1000 differentially expressed genes from two sAML stages relative to their chronic MF stage(s) with violin plots highlighting gene expression of shared transcription factors at sAML vs MF stage(s). d) ChIP tracks of key transcription factors identified from scRNA-seq showing occupancy at the Mus musculus Dusp6 locus across various tissue samples. e) Heatmap of pearson correlations between DUSP6 and top, shared candidates across five databases. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. f) KLF2 identified as the top correlating gene, and KLF1 identified among the bottom 10 correlating genes, with DUSP6 in patient 381812 (MF and sAML) scRNA-seq.
