Extended Data Fig. 9: Acetylation of c-Myc in Lys148 after inhibition of HDAC6. | Nature Cancer

Extended Data Fig. 9: Acetylation of c-Myc in Lys148 after inhibition of HDAC6.

From: Network-based assessment of HDAC6 activity predicts preclinical and clinical responses to the HDAC6 inhibitor ricolinostat in breast cancer

Extended Data Fig. 9: Acetylation of c-Myc in Lys148 after inhibition of HDAC6.The alternative text for this image may have been generated using AI.

a) MA plots showing the peptides upregulated upon HDAC6 knockout (above) and HDAC6 catalytic domain 2 mutants (below). In the MA plots, each dot represents a peptide. The significantly upregulated peptides were identified by fold change > 1.5 and p-value < 0.05 and highlighted in red. P value was estimated by two-tailed t test. (b) Scatter plot showing the correlation of the Z-score of the comparison between HDAC6 KO and wild type with that of the comparison between HDAC6 mutant and wild type. The curve was fitted by stat_smooth algorithm using lm smoothing method and y~x formula. The correlation coefficient (R) and P value were estimated using two-tailed Spearman correlation test. Each dot represents a peptide. For a and b the N = 2 independent proteomic replica studies per cell line. c) The western blot shows the accumulation of ac-K148-c-Myc after HDAC6 is inhibited by ricolinostat in MDA-MB-453 and SK-BR-3 BC lines. WT-blot results were reproduced n = 3 times from independent experiments.

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