Extended Data Fig. 4: Dysfunctional phenotype of T cells in iKRAS PDAC tumors identified by single cell gene expression profiling. | Nature Cancer

Extended Data Fig. 4: Dysfunctional phenotype of T cells in iKRAS PDAC tumors identified by single cell gene expression profiling.

From: Targeting T cell checkpoints 41BB and LAG3 and myeloid cell CXCR1/CXCR2 results in antitumor immunity and durable response in pancreatic cancer

Extended Data Fig. 4: Dysfunctional phenotype of T cells in iKRAS PDAC tumors identified by single cell gene expression profiling.The alternative text for this image may have been generated using AI.

A. Representative genes and functional markers used for identification of T cell clusters. B. Heatmap of two CD4+ and four CD8+ T cell clusters with unique signature genes. C. Cell cycle scoring for two CD4+ and four CD8+ T cell clusters. D. Relative expression of select genes in CD8+ T cells as a function of pseudotime from Monocle2 inferred trajectory. Each point corresponds to a single cell, colored by CD8+ T cell cluster. Lines represent average expression at that location in the trajectory. E. Quantification of immune checkpoint expression on infiltrating CD4+ and CD8+ T cells in iKRAS tumors (n = 3 biological replicates), assessed by flow cytometry and analyzed by FlowJo.

Source data

Back to article page