Extended Data Fig. 3: KAT8 interacts with IRF1.
a, Coomassie blue staining of an SDS-PAGE gel showing the proteins from the nuclear lysates of A375 cells expressing doxycycline-inducible V5-TurboID-KAT8 that pulled down with the streptavidin beads before subject to mass spectrometry analysis. See Methods for details of proximity labeling assay. b, Western blot analysis of PD-L1 expression in A375 cells expressing sgRNAs targeting IRF1 with or without 100 U/mL IFN-γ treatment for 6 h. c,d, A375 cells expressing BirA* (BirA R118G mutation)-fused KAT8 (c) or IRF1 (d) were treated with or without 100 U/mL IFN-γ in the presence of 50 μM biotin for 24 h. Cell lysates were immunoprecipitated with streptavidin beads and blotted with the indicated antibodies. e, HEK293T cells were transfected with the indicated constructs for 24 h. The cell lysates were immunoprecipitated with streptavidin or anti-MYC beads and blotted with the indicated antibodies. The experiment in a was performed once. The experiments in b-e were repeated three times with similar results.
