Extended Data Fig. 4: KAT8-IRF1 forms membrane-less condensates. | Nature Cancer

Extended Data Fig. 4: KAT8-IRF1 forms membrane-less condensates.

From: Disrupting the phase separation of KAT8–IRF1 diminishes PD-L1 expression and promotes antitumor immunity

Extended Data Fig. 4

a, Structured illumination microscopy (SIM) analysis of mEGFP-KAT8 and IRF1-mCherry localization in 143B cells. b, Live-cell images of 143B cells expressing mEGFP-KAT8 and IRF1-mCherry with DiD staining. c, 143B cells expressing TagBFP-KAT8 and IRF1-mCherry were stained with anti-fibrillarin, anti-coilin or anti-PML antibodies. d, Indicated purified proteins used for in vitro LLPS assays were analyzed by SDS-PAGE and stained with Coomassie blue. e, Droplet formation was analyzed in purified mEGFP-KAT8 WT/C316S, IRF1-mCherry at room temperature in the presence of 150 mM NaCl and 10% PEG 8000. The concentration of each protein was 10 μM. Hoechst 33342 was used for staining nuclei. Scale bars in a,c,e indicate 5 μm. Scale bar in b indicates 10 μm. The experiments in a-e were repeated three times with similar results.

Back to article page