Extended Data Fig. 3: PDGF-C levels are upregulated in aged and fibrotic lungs.
a, Additional quantification for Fig. 2a. Left, number of metastatic deposits in the lungs.. Right, percentage metastatic tumour area in the lungs. b, Additional images of the experiment presented in Fig. 2b illustrating EdU+ tumour cells in young and aged mouse lungs. Tumour cells were identified by staining with an antibody against HMGA2. White arrowhead points to single dormant tumour cell (scale bars; 100 µm, upper images; 250 µm, lower images). c, Independent repeat of the experiment presented in Fig. 2c. TSAE1 tumour cells were injected intravenously into the tail vein of young (n = 5 mice) or aged (14-month, n = 5 mice) BALB/c mice. Percentage metastatic tumour burden in the lungs (day 15). d, Associated with Fig. 2d. Principal component analysis (PCA) of young (grey triangles) and aged (light red circles) NTB mouse lungs (RNA-seq data). Average values per group are shown with black and dark red shapes for young and aged lungs, respectively. e,f, BALB/c mice were treated with vehicle or bleomycin (see Methods for schedule) and culled 7 or 14 days after treatment ended (n = 3 mice per group). e, Representative images of αSMA and F4/80 (7 days post-treatment) and picrosirius red (PSR) (14 days post-treatment) staining in lungs (scale bar, 250 µm). f, Representative images of αSMA (green), PDGF-C (red) and DAPI (blue) staining in lungs of mice culled 14 days after treatment ended (scale bar, 150 µm). g, RNAscope analysis of Pdgfc expression in young (12-week), aged (13-month) or fibrotic (bleomycin-treated) lungs (from experiments shown in Fig. 2d,h). Lung sections were counterstained for αSMA (cyan). Scale bar, 25 µm. a,c, Data are presented as mean values ±SEM; a (left),c, two-tailed t-test; a (right), two-tailed Mann-Whitney U-test.
