Extended Data Fig. 9: Pdgfc knockdown in tumour cells has limited effects on the primary tumour stroma but reduces αSMA+ CAF numbers in metastatic deposits.
a,b, Representative immunostaining of shNTC (1,2) and shPdgfc (1,5) TSAE1 primary tumours from Fig. 4f. a, Sections stained for the endothelial marker endomucin (red), pericyte marker endosialin (green) and DAPI (blue) (scale bar, 100 µm). Brightness/contrast adjustment was performed on individual channels (ImageJ; all panels adjusted the same) before merging. b, αSMA immunohistochemistry (scale bar, 500 µm). c, Scoring of αSMA positivity of the lung metastases from Fig. 5e,f. Each bar represents an individual mouse. Scores calculated based on percentage of the metastatic deposit that was αSMA-positive: 0, 0%; 1, <20%; 2, 20-50%; 3, >50%. Note, two HRM1-shPdgfc mice had no detectable lung deposits, labelled as ‘x’.
