Extended Data Fig. 3: Immunohistochemical (IHC) staining and Western blot analysis of Cal-33 xenograft tumors. | Nature Cancer

Extended Data Fig. 3: Immunohistochemical (IHC) staining and Western blot analysis of Cal-33 xenograft tumors.

From: Specific regulation of BACH1 by the hotspot mutant p53R175H reveals a distinct gain-of-function mechanism

Extended Data Fig. 3: Immunohistochemical (IHC) staining and Western blot analysis of Cal-33 xenograft tumors.The alternative text for this image may have been generated using AI.

a-d, IHC staining for 4-HNE (a), SLC7A11 (b), Ki67 (c) and TUNEL (d). Bar scale=20 μm. e-h, Quantification for 4-HNE (e), SLC7A11 (f), Ki67 (g) and TUNEL (h) staining. n = 5 fields from 5 tumors (each group). P values in e (from left to right): 0.0018; 0.0007. P values in f (from left to right): 0.0003; 0.0001. P values in g (from left to right): 0.5447; 0.5447. P values in h (from left to right): 0.9505; 0.7634. i, Western blot analysis of GPX4 expression. n = 3 tumor samples for each genotype. 4-HNE, SLC7A11 and Ki67 IHC staining was quantified by using the immunoreactive score (IRS) system and TUNEL staining were quantified with percentage of positive cells (see Methods for details). Two-tailed Student’s t-test were used for statistical analysis. ns, not significant, **p < 0.01; ***p < 0.001. Data represent mean + S.E.M.

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