Extended Data Fig. 3: PARP1 protein levels positively correlate with USP15 in TNBC.
(a) The Hematoxylin and eosin (H&E) staining analysis of the breast cancer tissue specimens, and the associated normal tissue samples. (b) Western blot analysis of PARP1 and USP15 expression in 14 other sets of TNBC tissues and the corresponding adjacent normal mammary tissues (4 of these tissues are shown in Fig. 3c). (c) Western blot analysis of PARP1 and USP15 expression in the other 12 sets of non-TNBC tissues and the corresponding adjacent normal mammary tissues (4 of these tissues are shown in Fig. 3e). (d–e) The comparison of PARP1 or USP15 protein levels between 90 sets of breast cancer tissues, namely, 54 TNBC tissues and 36 non-TNBC tissues, and the corresponding adjacent normal mammary tissues by the immunohistochemistry assay. Data are expressed as mean ± s.e.m.. (f) Representative immunohistochemistry images of USP15 and PARP1 expression in breast cancer tissues and adjacent normal mammary tissues. (g) Comparison of PARP1 mRNA levels between TNBC tissues and the corresponding adjacent normal mammary tissues. mRNA was extracted from clinical tissues and analyzed by quantitative PCR, n = 3 technical replicates. P values are indicated (d–e), and statistical significance was determined by Student’s t test. Experiments were repeated three times independently with similar results; data of one representative experiment are shown (a–c, f–g).
