Extended Data Fig. 6: USP15-PARP1 axis promotes BER to accelerate TNBC growth.
(a) Analysis of PARP1 and USP15 levels in different groups of HCC70 cells. (b, c) Analysis of MDA-MB-231 xenograft growth in vivo. Different groups of MDA-MB-231 cells were engrafted into the right flank of immunocompromised mice. At different time points post injection, tumors were measured, and tumor volumes were calculated and recorded. Tumor volumes are shown in (b). Tumor weights at the experimental endpoint are shown in (c). A representative picture of the tumors is shown in (c). Data are expressed as mean ± s.e.m., n = 5 mice for each group. (d) Analysis of 8-oxoguanine and γH2AX positive cells in MDA-MB-231 xenograft samples. Data are expressed as mean ± s.e.m., n = 5 for each group, each point represents a xenograft tumor, the tumors used for analysis in each experiment were from a single replicate. n.s., not significant. P values are indicated (b-d). Statistical significance was determined by one-way ANOVA test followed by Tukey’s multiple comparison (c-d), or two-way ANOVA test followed by Tukey’s multiple comparison (b). Experiments were repeated three times independently with similar results; data of one representative experiment are shown (a).
