Extended Data Fig. 3: Single-cell transcriptome analysis of the 22Rv1-WT cell line and 22Rv1 liver metastasis.
a, Uniform Manifold Approximation and Projection (UMAP) reduced dimension plots shows 22Rv1-WT consists of different clusters. The majority of 22Rv1 liver metastasis cells grouped as a single cluster, albeit this scRNA analysis was limited due to the number of viable cells of the sequenced liver metastasis (n = 197 cells) compared with 22Rv1-WT cell line (n = 3467 cells). b, Single-cell RNA expression of FOLH1, AR, HOXB13, LAT1 and NE markers over the UMAP representation of the map. The expression of LAT1 in Cluster 3 shows PSMA-low subpopulation of 22Rv1-WT cells are not LAT1 positive. This observation implies there is a heterogeneity within the PSMA-low cell populations. c, Unsupervised clusters were annotated as three clusters with distinct FOLH1 levels. d, Expression of FOLH1 in identified clusters. e, Stacked barplot displaying percentage of each cluster in 22Rv1-WT and 22Rv1 liver metastasis. Notably, only 10.9% of 22Rv1-WT belong to PSMA-low Cluster 3. However, more than 58% of 22Rv1 liver metastasis are within Cluster 3. In d, the data were analyzed by one-way ANOVA followed by Šídák’s multiple comparison tests. In a-c, data presented is based on data from a single experiment.
