Extended Data Fig. 6: Overlap or proximity of chromatin accessible regions with MM-preferential dependencies.
a, Plot of stitched regions of chromatin accessibility with average ATAC-seq signal (RPPM) across 22 MM cell lines shown in gray. Black lines denote the inflection point that denotes super-accessible (SA) regions. Regions within 100 kb of MM-preferential dependencies are denoted by red tick marks on the bottom and the odds ratio (OR) and P-value (two-sided Fisher’s exact test) of enrichment of MM-preferential dependencies found near super-accessible regions are shown. b, Heatmap of chromatin accessible regions within 100 kb of MM-preferential dependencies across 22 MM cell lines. c-f, Genomic plots of ATAC-seq for select examples of MM-dependencies (PRDM1, IRF4, POU2AF1, UBE2J1) that overlap with super-accessible (SA) regions. Each cell line is shown in a transparent gray and the average is shown in black. Note the proximity of IRF4 and DUSP22 and the multiple prominent areas of accessible chromatin within intronic regions of DUSP22. g, Hierarchical clustering of MM cell lines based on their CERES scores for MM preferential dependencies. MM cell lines are annotated for their status for genomic events, such as translocations targeting CCND1, CCND2, CCND3, MAF, MAFB, MMSET/NSD2, mutations for KRAS or NRAS, loss-of-function for TP53; or the functional status of their dependence (based in CRISPR data) on either MAF or MAFB.
