Fig. 3: HNRNPA2B1 binds and stabilizes the long NQO1 isoform and increases NQO1 expression. | Nature Cancer

Fig. 3: HNRNPA2B1 binds and stabilizes the long NQO1 isoform and increases NQO1 expression.

From: A sense-antisense RNA interaction promotes breast cancer metastasis via regulation of NQO1 expression

Fig. 3: HNRNPA2B1 binds and stabilizes the long NQO1 isoform and increases NQO1 expression.

a, Read distribution in HNRNPC iCLIP and HNRNPA2B1 CLIP experiments relative to annotated poly(A) sites and NQO1-AS complementary region along the NQO1 3′-UTR. b, NQO1 mRNA decay rate in MDA-LM2 cells with and without knockdown of HNRNPA2B1 (ref. 21). n = 4 independently treated cell cultures. c, RT–qPCR showing NQO1 expression in MDA-LM2 cells with and without knockdown of HNRNPA2B1. n = 3 independent cell cultures. d, RT–qPCR showing NQO1 long and short isoform fractions in HNRNPA2B1 knockdown MDA-LM2 cells, normalized by the same measurements in cells transfected with a nontargeting control. n = 3 independent cell cultures. e, Relative stability of long and short NQO1 isoforms with and without HNRNPA2B1 knockdown in MDA-LM2 cells. n = 3 independent cell cultures. f, Summary of the mechanism by which overexpression of NQO1-AS causes NQO1 upregulation in highly metastatic breast cancer cells. All P values were calculated using a one-tailed Mann–Whitney U-test.

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