Extended Data Fig. 3: CD8 + pTRT are expanded predominantly in tumor tissue and can be detected by flow cytometry as CD39 + CCR7low cells.
a, Dot plot showing the relationship between the abundance of cluster C3 among CD8 + T cells and the frequencies of CD39 + CCR7low cells among all CD8 + T cells in six brain tumors profiled by FCM and scRNA-seq. Significance determined with linear regression. b, CD8 + T cells in pTRT-high tumors are highly abundant and clonally expanded. Representative alluvial plots visualizing the frequency of the top 15 T cell receptor (TCR) clones within three patients (pTRT-high BrM, pTRT-low BrM, and a glioma patient) in the blood and tumor, respectively. Each clone within an individual patient sample is annotated with a unique color.
