Extended Data Fig. 7: eFT508 inhibits translation of Hgf, Spp1 and Bgn and impairs PMN-MDSCs migration in prostate cancer.
a, Hgf, Spp1 and Bgn mRNA levels in polysomes-bound mRNAs and total mRNAs fraction in prostate cancer of eFT508-treated and vehicle-treated Ptenpc−/−;Trp53pc−/− mice determined by qRT- PCR (n = 3 mice in each group). Data are mean ± SD. Statistical analysis (two-tailed ratio paired t-test). b, Densitometry of BGN, SPP1 and HGF protein expression levels in prostate cancer of eFT508-treated or vehicle-treated Ptenpc−/−;Trp53pc−/− mice (BGN and HGF, n = 3 mice ; SPP1, n = 4 mice). Data are mean ± SD. Statistical analysis (unpaired two-sided Student’s t-test). c, Ifng, Granzyme B (GrzmB), Perforin (Prfn) and FoxP3 mRNA levels in prostate cancer of eFT508-treated compared to vehicle-treated Ptenpc−/−;Trp53pc−/− mice determined by qRT- PCR (Ifng, GrzmB and Prfn, n = 3 mice, Foxp3 n = 4 mice). Data are mean ± SD. Statistical analysis (two-tailed ratio paired t-test). d, Number of migrated MDSCs tested in a transwell migration assay: MDSCs, previously exposed to 10% FBS, vehicle, 100 nM or 500 nM eFT508-treated Ptenpc−/−;Trp53pc−/− (RapidCap)-derived conditioned media for 24 hours, were allowed to migrate through a 5 μm-transwell to the bottom well for 6 hours toward Pten−/−;Trp53−/− (RapidCap)-derived conditioned media. The number of migrated cells was determined by flow cytometric analysis. Experiment in technical replicates performed twice with similar results. e, Number of migrated MDSCs tested in a transwell migration assay: MDSCs were allowed to migrate through a 5 μm-transwell to the bottom well for 6 hours toward 0.1% FBS media, vehicle, 100 nM or 500 nM eFT508-treated Pten−/−;Trp53−/− (RapidCap)-derived conditioned media. The number of migrated cells was determined by flow cytometric analysis. Experiment in technical replicates performed twice with similar results.
