Extended Data Fig. 1: Polysome profiling analysis in Pten-null-driven prostate cancer. | Nature Cancer

Extended Data Fig. 1: Polysome profiling analysis in Pten-null-driven prostate cancer.

From: The Akt/mTOR and MNK/eIF4E pathways rewire the prostate cancer translatome to secrete HGF, SPP1 and BGN and recruit suppressive myeloid cells

Extended Data Fig. 1

a, Polysome profiles of wild-type prostate, Ptenpc−/−, Ptenpc−/−;TMPRSS2/Ergpc+/+, Ptenpc−/−;CDCP1pc+/+, Ptenpc−/−;Timp1−/− and Ptenpc−/−;Trp53pc−/− prostate cancer. RNA-seq was performed on polysome-bound RNAs and total RNA derived from the prostate of three mice for each genetic background for a total of 18 samples. b, PCA plots of total (T) and polysomal RNA (P) fractions for each analyzed genetic background (n = 3 mice for each genetic background for a total of 18 samples). c, PCA plots of total and polysomal RNA fractions for each genetic background analyzed, corrected for the batch effect (n = 3 mice for each genetic background for a total of 18 samples). d, Scatter plots of fold-changes for polysome-associated and total mRNA levels for the comparisons between the indicated genetic backgrounds and wild-type prostates showing mRNAs with upregulated translation efficiency (red), downregulated translation efficiency, buffering up (pink), buffering down (blue) (n = 3 mice for each genetic background for a total of 18 samples). Details are provided in Supplementary Table 1 and Supplementary Table 2.

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