Extended Data Fig. 9: The absence of Siglece enhances the efficacy of ICB therapy in murine GBM model.

(a) Schematic illustration of ICB therapy. WT or Siglece−/− mice were subcutaneously inoculated with CT2A glioma cells and the mice bearing tumors were administered intraperitoneally with anti-mouse PD-1, anti-mouse PD-L1 or left untreated on days 12, 16, 20, and 24. Tumor growth was monitored. (b, c, d and e) The subcutaneous tumor volume (b, d) and overall survival (c, e) of WT and Siglece−/− mice treated with anti-PD-1 (b, c), anti-PD-L1 (d, e) or left untreated after inoculation of CT2A cells. The numbers of mice are as indicated. Data are means ± SEM (b, d). Two-sided log-rank test. (f) Schematic illustration of Siglec-E-Fc therapy. WT mice were subcutaneously inoculated with CT2A glioma cells and the mice bearing tumors were administered intraperitoneally with Siglec-E-Fc or IgG. Tumor growth was monitored. (g) The subcutaneous tumor volume of WT mice treated with Siglec-E-Fc or IgG after inoculation of CT2A cells. The numbers of mice are as indicated. Data are means ± SEM. Two-sided log-rank test.