Extended Data Fig. 2: Expression heterogeneity in the paratumor and tumor core regions of human GBM determined by ST.
(a) Heatmap depicting pairwise correlations of the indicated expression programs derived from 34 ST datasets (18 paratumor sections and 16 tumor core sections). Clustering identified six coherent expression programs across the ST sections. (b) Expression scores of gene sets analyzed by ST for representative samples. Non-malignant and malignant cells are indicated according to the CNV analysis results. See also Supplementary Fig.s 1 and 2. (c) Plots showing hypoxia, immune inflammatory and angiogenesis scores for ST spots in paratumor. Box limits represent the first quartile and third quartile, the line inside the box represent s the median, and the whiskers extend from the box to show the 5th percentile to the 95th percentile (n = 44,629 spots over 34 samples from 18 patients). Two-sided Student’s t-test. (d) Volcano plot showing differentially expressed genes [−log10(adjusted P) > 5, log2(FC) > 0.5] in the tumor core vs. paratumor regions in human GBM.
