Fig. 1: Landscape of advanced ovarian cancer via scRNA-seq of five sites.
a, Overall study design with flowchart of sample collection and single-cell analysis of OC by 10x Genomics sequencing. n = 14 patients with OC who were responsive or nonresponsive to platinum-based chemotherapy were recruited to our study. In total, n = 39 samples, including n = 6 PB, n = 5 PLN, n = 13 Pri.OT, n = 5 matched Met.Ome and n = 10 ascites samples were analyzed. Each dot corresponds to one sample, colored by sample types. Red triangle, orange triangle, dark red circle, dark red triangle, green triangle represent blood, ascites, primary tumor, omentum metastases and pelvic lymph node, respectively. b, Uniform Manifold Approximation and Projection (UMAP) plot showing 14 clusters of n = 10 patients with HGSOC identified by integrated analysis. Each dot corresponds to a single cell, colored by clusters. NK, natural killer; HSC, hematopoietic stem cell. c, Heat map depicting expression levels of selected highly expressed genes (including marker genes) across major clusters of HGSOC. Rows represent genes and columns represent clusters. d, Tissue preference of each major cluster in HGSOC estimated by Ro/e. e, UMAP plots showing the distinct cell composition of five different sample sites in patients with HGSOC. For b–e, a total of n = 31 HGSOC samples, including n = 5 PB, n = 4 PLN, n = 10 Pri.OT, n = 4 Met.Ome and n = 8 ascites samples were analyzed.
