Extended Data Fig. 3: Gene expression, tissue distribution and clonal types of T-cell clusters.
a, Heatmap showing selected highly expressed genes of 12 T cell clusters. Rows represent genes and columns represent clusters. b, UMAP plots showing selected marker genes of 12 T cell clusters. Each dot corresponds to a single cell. c, Tissue distribution of each T cell cluster, colored by different tissues (upper) and clusters (lower). d, Representative flow-cytometric plots, and summary data of frequency of CD25+ CD127+ CD4+ T cells (Treg) and PD-1+ CD8+ T cells in the microenvironment of n = 5 primary tumors, n = 5 matched omentum metastasis and n = 5 ascites samples from 5 HGSOC patients. P values were determined by paired one-sided t-test. *P < 0.05, **P < 0.01, ***P < 0.001. e, Violin plots showing the differentially expressed genes of two CD8+ Tem clusters with distinct tissue preference. CD8-ANXA2 was enriched in tumor sites and CD8-GZMK was enriched in ascites. f, The proportion of each T cell clonotype in each T cell cluster (upper) and tissue distribution of each clonotype (lower), colored by clonotypes. g, The proportion of clonal T cells in each cluster and different tissues, each color represents a patient diagnosed with HGSOC. AS: ascites, PT: primary ovarian tumor, MT: metastatic ovarian tumor, PB: peripheral blood, LN: lymph node. For a-c and e-g, data were summarized from all n = 31 HGSOC samples.
