Fig. 3: Characterization and dynamics of CD4+ T cells in HGSOC.
a, Clonal expansion, migration and transition potential of CD4+ T cells quantified by STARTRAC indices. Indices were quantified for each n = 9 patient with more than two matched samples. Center line indicates the median value, lower and upper hinges represent the 25th and 75th percentiles, respectively and whiskers denote 1.5 × interquartile range. *P < 0.05, **P < 0.01, ***P < 0.001; permutation test (exact P values are provided in source data). b, PAGA analysis of CD4+ T cells. Each dot represents a T cell cluster. In total n = 31 HGSOC samples were used for analysis. c, Heat map showing the developmental transition potential between CD4+ T cells quantified by pairwise STARTRAC-tran indices. The red box represents the transition between TCM and other CD4+ T cells. d, The distribution of clonal clonotypes in indicated CD4+ subsets derived from ascites and two tumor sites. e, Bar plots showing proportions of shared TCRs between TCM (T02) and TH1-like cells (T05) corrected by cell numbers of TCM (T02) in ascites, related to Extended Data Fig. 5e. f, Frequency of TH1-like cells as a proportion of all CD4+ T cells in n = 4 Met.Ome and n = 10 Pri.OT samples from ten patients with HGSOC. Center line indicates the median value, lower and upper hinges represent the 25th and 75th percentiles, respectively and whiskers indicates min to max. *P < 0.05, **P < 0.01, ***P < 0.001; unpaired two-sided t-test. g, Sketch map showing the dynamics of CD8+ T cells (top) and CD4+ T cells (bottom) between ascites and two tumor sites. For a,c–e, data were summarized from all n = 30 HGSOC samples except for the primary tumor sample of HGSOC7.
