Extended Data Fig. 4: Myeloid cell specific deletion of Kdm6b does not affect myeloid cell abundance.
From: Myeloid-specific KDM6B inhibition sensitizes glioblastoma to PD1 blockade
a, Schematic representation demonstrating generation of the LysMcreKDM6Bfl/fl genetic murine model. b, Representative image of an agarose gel showing bands depicting PCR amplified DNA from Kdm6b deleted homozygous mice (single 400 bp band), Kdm6b deleted heterozygous mice (both 368 and 400 bp bands), and control homozygous mice (single 368 bp band). Data is representative of two independent experiments. c, t-SNE plots and box and whisker plots depicting the identity and abundance of different immune cell populations present in the indicated anatomical locations in control and LysMcreKDM6Bfl/fl mice as determined from CyTOF analysis. n = 3 female mice/group. Two-tailed Student’s t test was performed (ns= non-significant). Whiskers represent minimum to maximum values.
