Extended Data Fig. 5: Myeloid specific deletion of Kdm6b alters the abundance of intratumoral immune cells.
From: Myeloid-specific KDM6B inhibition sensitizes glioblastoma to PD1 blockade
a, Heatmap showing the expression of genes of interest in the different Cd45+ immune cell clusters (shown in Fig. 3c). b, Bar graphs depicting the frequencies of the different intratumoral immune cell subsets in control and LysMcreKDM6Bfl/fl mice. c, Bar graph representing the ratio of intratumoral CTLs and Tregs in control and LysMcreKDM6Bfl/fl mice as determined from scRNA seq. d, Violin plots depicting the expression level of Lyz2 in different immune cell clusters in control and LysMcreKDM6Bfl/fl mice. Data representative of two independent scRNA seq experiments. e–g, Box and whisker plots representing the flow cytometry analysis of abundance of different immune cell populations present in PBMC (e), lymph nodes (f) and spleen (g) derived from control and LysMcreKDM6Bfl/fl GBM tumor bearing female mice (n = 3 biologically independent samples/group for (E), n = 4 biologically independent samples/group for f and g. Two-tailed Student’s t-test was performed. Whiskers represent minimum to maximum values.
