Extended Data Fig. 8: Absence of Kdm6b inhibits acquisition of an immune-suppressive phenotype in BMDMs.
From: Myeloid-specific KDM6B inhibition sensitizes glioblastoma to PD1 blockade
a, Representative gating strategy on FlowJo for analysis of flow cytometry data showing GL261 phagocytosis by BMDMs (shown in Fig. 6j). b, Box and Whisker plots showing the concentration of the indicated cytokines in 24 hour culture supernatants of M2, M1 and M0 BMDMs derived from control and LysMcreKDM6Bfl/fl male mice(n = 6 biologically independent samples/condition). Data is representative of 2 independent experiments. Two-tailed Student’s t-test was performed. Whiskers represent minimum to maximum values. c, Relative average expression of indicated genes in M2 and M1 BMDMs derived from control and LysMcreKDM6Bfl/fl male mice (n = 3 biologically independent samples for control M1 BMDMs and n = 4 for LysMcreKDM6Bfl/fl M1 BMDMs). Two-tailed Student’s t-test was performed. Whiskers represent minimum to maximum values. d,e, Concentration of IL-6 and IL-10 in the supernatants of BMDMs derived from control and LysMcreKDM6Bfl/fl male mice following efferocytosis and subsequent LPS stimulation (n = 4 biologically independent samples/condition). Two-tailed Student’s t-test was performed. Data is representative of two independent experiments. Whiskers represent minimum to maximum values. f,g, Box and Whisker plots demonstrating the relative gene expression of INOS and IL10 in BMDMs derived from control and LysMcreKDM6Bfl/fl male mice following efferocytosis and subsequent LPS stimulation (n = 3 biologically independent samples for control BMDMs and n = 4 LysMcreKDM6Bfl/fl BMDMs for (F), n = 4 biologically independent samples for control BMDMs and n = 3 LysMcreKDM6Bfl/fl BMDMs for G). Two-tailed Student’s t-test was performed. Whiskers represent minimum to maximum values.
