Fig. 6: Notch1-driven changes in vitamin A metabolism occur during precachexia.
From: Endothelial Notch1 signaling in white adipose tissue promotes cancer cachexia
a, RT–qPCR analysis of Aldh1 mRNA levels in AT-ECs from KPC mice (n = 5–6 animals per group). b, AT-EC Aldefluor activity in KPC mice versus in non-tumor-bearing control mice (n = 5–6 animals per group). c, RT–qPCR analysis of Igfbp3 mRNA levels in AT-ECs (n = 5–6 animals per group). d, RT–qPCR analysis of Il33 mRNA levels in AT-ECs (CD31+CD45–) from KPC and control mice (n = 6 animals per group). e, Aldh1a2 mRNA expression in macrophages (CD45+CD11b+F4/80hi) from KPC mice (n = 6 animals per group). f, Flow cytometry histogram plot of Aldefluor activity in control and KPC macrophages. g, Analysis of ALDH activity in myeloid cells, including macrophages (CD45+CD11b+F4/80hi), monocytes (CD45+CD11b+Ly6C+), eosinophils (CD45+CD11b+SiglecF+) and neutrophils (CD45+CD11b+Ly6G+), measured by flow cytometry in sWAT from KPC and control mice (n = 5–6 animals per group). h,i, CD45+ immune cells (h) and myeloid cell populations (i) quantified as a percentage of total living cells (DAPI–) in KPC and control sWAT (n = 5–6 animals per group). Data shown represent mean ± s.e.m. and were analyzed by Mann–Whitney test (a) or unpaired, two-sided t-test with Welch correction (b–e and g–i). Experiments in a, c and d were performed twice with consistent results. Shown are data from one representative experiment.
