Extended Data Fig. 1: Increased glucose uptake upon Pdcd1 loss.
a, Percentage of eGFP-expressing cells as determined by flow cytometric analysis of peripheral blood. A cross indicates death of the animal. Individual data points are shown (n = 3 mice per genotype). TAM, tamoxifen. b, Remaining glucose in cell culture supernatants. ITK-SYK-expressing T cells were sorted by FACS for eGFP+ from the spleens of acutely induced ITK-SYKCD4-creERT2 (‘Pdcd1+/+’) and ITK-SYKCD4-creERT2;Pdcd1−/− mice (‘Pdcd1−/−‘) and cultured in vitro for four hours. Next, the cell culture supernatants were harvested, and glucose concentrations were determined by colorimetric measurements. All animals received single-dose intraperitoneal injections of tamoxifen five days before the experiment (n = 6 and n = 4 mice per genotype). Data was normalized to viable cell number. P=two-sided Student’s t-test. Shown are the mean ± SD and individual data points. a, Representative results from 8 independent experiments with three biological replicates per group. b, Representative results from 2 independent experiments.
