Extended Data Fig. 8: Molecular cytogenetic results from metaphases of healthy and neoplastic specimens.
From: Somatic evolution of marine transmissible leukemias in the common cockle, Cerastoderma edule
a–b, FISH of 28S ribosomal DNA (rDNA; violet), 5S rDNA (red) and H3 histone gene (green) probes mapped onto a metaphase plate of a healthy specimen of C. edule and its corresponding karyotype with 2n = 38 chromosomes. As previously described80, up to five chromosome pairs hold subtelomeric clusters of 5S rDNA on their long arms, while 28S rDNA and histone H3 probes hybridize to the short arm of subtelocentric chromosomes. c–d, FISH mapping of the probes above onto example neoplastic metaphases, revealing abnormal location and number of these clusters. Scale bars, 10 µm.
