Extended Data Fig. 9: Differential expression results.
(a) Hierarchical clustering of all RNA sequenced samples by the expression of the top 100 most significant genes expressed in each specific healthy tissue relative to all other tissues, with heatmap of normalized relative gene expression for each gene. MarBTN (BTN) clusters most closely with hemocytes (heme), supporting principal-component analysis results. (b) Volcano plot of polymerase genes expression (n = 28) for MarBTN (n = 5) compared with non-hemocyte tissues (n = 15: 5 tissues for 3 clams). (c) Normalized expression, in reads per gene, of four genes with detectable positive dN/dS for MarBTN (n = 5), hemocytes (n = 5), and non-hemocyte tissues (n = 15: 5 tissues for 3 clams). Bars display mean, error bars display standard deviation, and differential expression comparison results displayed as * = p<0.01, ** = p<1e-7, ns = not significant. Exact p-values are 9.6e-1, 3.7e-2, 1.8e-1, 8.1e-3, 7.4e-1, 1.5e-8, 6.0e-3 and 3.1e-1 respectively.
